Antioxidant, anti-inflammatory and antinociceptive activities of methanolic extract of Justicia secunda Vahl leaf

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Samuel Onoja Maxwell Ezeja Yusuf Omeh Beatrice Onwukwe

Abstract

Background of study: Plants used for traditional medicine contain a wide range of substances
which can be used to treat various infectious diseases.
Aim: The study evaluated the in vitro antioxidant, antinociceptive, and anti-inflammatory activities
of the methanolic extract of Justicia secunda Vahl leaf.
Methods: The acute toxicity was performed with up and down method and the highest dose used was
2 g/kg. The anti-inflammatory activity was evaluated using the carrageenan and formalin-induced
paw edema models, and antinociceptive activity was evaluated using acetic acid-induced writhing
reflex and tail flick test models while the antioxidant activity was evaluated using 2,2-diphenyl-2-
picryl hydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) photometric assay.
Results: The extract was well tolerated as no signs of toxicity or death were noticed during the period
of observation. The extract produced a concentration dependent increase in antioxidant activities in
both DPPH and FRAP models. The extract produced its optimum activity at 400 lg/ml in both
DPPH (54.07%) assay and FRAP (1.58 lM) assay. The extract produced significant (P < 0.05)
dose-dependent increase in both anti-inflammatory and antinociceptive activities. The antinociceptive
and anti-inflammatory activities of the extract (0.4 g/kg) were comparable with the reference
drugs (aspirin and pentazocine) used in the study.
Conclusion: This study suggests that J. secunda possesses anti-inflammatory, antinociceptive and
antioxidant activities and also provide the pharmacological basis for its uses in traditional medicine
for these purposes.
2016 Alexandria University Faculty of Medicine. Production and hosting by Elsevier B.V. This is an
open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Author Biographies

Samuel Onoja

Department of Veterinary, Physiology, Pharmacology, Biochemistry and Animal Health and Production, College of
Veterinary Medicine, Micheal Okpara University of Agriculture, PMB 7267 Umudike, Abia State, Nigeria

Maxwell Ezeja

Department of Veterinary, Physiology, Pharmacology, Biochemistry and Animal Health and Production, College of
Veterinary Medicine, Micheal Okpara University of Agriculture, PMB 7267 Umudike, Abia State, Nigeria

Yusuf Omeh

Department of Biochemistry, College of Natural Sciences, Micheal Okpara University of Agriculture, PMB 7267 Umudike, Abia State, Nigeria

Beatrice Onwukwe

Department of Biochemistry, College of Natural Sciences, Micheal Okpara University of Agriculture, PMB 7267 Umudike, Abia State, Nigeria