Evaluation of Interleukin 8 gene polymorphism for predicting inflammation in Indian chronic kidney disease and peritoneal dialysis patients

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Rajasree Shanmuganathan Kumaresan Ramanathan Giri Padmanabhan Bhooma Vijayaraghavan

Abstract

Background and aim: Previous studies have observed the association between inflammation
and chronic kidney disease (CKD). The role played by Interleukin 8 (IL8) gene polymorphism
has not been studied yet. Hence, the present study has been designed as the first attempt to identify
the possible associations between polymorphism of the IL-8 gene and patients with diabetic CKD
and on continuous ambulatory peritoneal dialysis (CAPD).
Materials and methods: A total of 150 participants were selected from a private nephrology outpatient
clinic. The subjects were divided into three groups: healthy individuals without any renal complications
(group 1, control, n=50), patients with diabetic chronic kidney disease of stages 3 and 4
(group 2, n= 50) and CAPD (group 3, n= 50). Blood deoxyribo nucleic acid (DNA) isolated from
the members of the study group, was confirmed by agarose gel electrophoresis and primers specific
for IL8 gene were designed, using primer3 software tool.
Results: Restriction digestion of the amplicons with Escherichia coli restriction enzyme I (EcoRI) ended
up in 203 base pairs (bp) band in control and 108 bp band in all diabetic and non-diabeticCKD. This indicated
the presence of polymorphismin +781 Cytosine/Thymine (C/T) of IL-8 gene in diabetic CKDand
CAPD patients. Statistical analysis of the distribution of frequencies of alleles C and T by chi square test
confirmed the presence of polymorphismat+781C/T of IL-8 gene in patient groups compared to control.
Conclusion: The polymorphismin+781C/T of IL-8 gene studied in thiswork suggests its possible role as
an inflammatory marker for both chronic kidney disease and CAPD.
2016 Alexandria University Faculty of Medicine. Production and hosting by Elsevier B.V. This is an
open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Author Biographies

Rajasree Shanmuganathan

CoRx Life Sciences And Pharmaceutical (CLAP) Private Limited, Tiruchirappalli 620020, India

Kumaresan Ramanathan

Department of Biotechnology, Periyar Maniammai University, Vallam, Thanjavur 613 403, India

Department of Biochemistry, Institute of Biomedical Science, College of Health Sciences, Mekelle University (Ayder
Campus), Mekelle, Ethiopia

Giri Padmanabhan

Kidney Care, C-50, 10th B Cross, Thillai Nagar, Tiruchirappalli 620 018, India

Bhooma Vijayaraghavan

Kidney Care, C-50, 10th B Cross, Thillai Nagar, Tiruchirappalli 620 018, India