Diagnostic lab

About the lab

  • The microbiology laboratory is a full service laboratory providing diagnostic services for patients with infections seen in inpatient, intensive care units and outpatient facilities of the Hospital.
  • The lab receives specimens from patients for isolating microorganisms that are, or may be, a cause of the illness, identifies the etiological agent and provides information about the in vitro activity of antimicrobial drugs against the microorganisms identified.
  • Therefore, it provides the clinician with information that would facilitate the treatment of patients with serious infectious diseases by appropriate antimicrobial therapy as soon as possible.

Staff members

  • Professor Manal Baddour
  • Assistant Professor Reem Harfoush
  • Assistant Professor Nesrine Fathi
  • Lecturer Noha Abo Seada
  • Lecturer Hadir Okasha
  • Lecturer Shams Arafa
  • Lecturer May Moheb
  • Lecturer Marwa Meheissen
  • Assistant Lecturer Sara Asser
  • Assistant Lecturer Rola Hashad
  • Assistant Lecturer Dina Kholeif
  • Demonstrator Yara Safwat
  • Demonstrator Amira Baz
  • Demonstrator Amany Refaat
  • Demonstrator Nehal Shahtout
  • Demonstrator Sara Abdel Aziz
  • Demonstrator Mayada Nabil
  • Demonstrator Gehad Mahmoud
  • Demonstrator Nancy Ouf
  • Demonstrator Ingy Nassar

Contact information

Department of Microbiology and Immunology
University of Alexandria Faculty of Medicine
Phone: 03-4861526/ 011 561 246 55
Email: Manal.baddour@alexmed.edu.eg

services

  1. Diagnostic:
    • The lab carries diagnostic tests for identification of bacterial agents causing infectious diseases, as well as antimicrobial susceptibility tests to define patterns of sensitivity to different antimicrobial classes based on the latest guidelines of CLSI. The tests performed by the lab are:
      1. Microscopic examination, culture and sensitivity of:
        • Urine samples
        • Stool samples
        • Sputum samples
        • BAL and miniBAL samples
        • Pus swabs and tissue samples
        • Blood samples (conventional culture and BACT Alert)
        • Aspirate samples (pleural, pericardial, peritoneal)
        • CSF samples
      2. Serological tests:
        • Widal test
        • Rose Bengal test
      3. Anaerobic cultures
    • Our diagnostic lab is pleased to announce that these tests can be performed now for anyone for a reasonable price using internationally approved methods, standards, equipment and guidelines and using the best expertise in the field to provide the highest quality analysis and results.
  2. Research:
    • The lab provides the necessary material, space and equipment for researchers to carry out bacteriological, serological and antimicrobial tests for research purposes.
    • Trained personnel (microbiology technicians) are available to assist in carrying out research.
    • Some thesis carried out in the lab for research purposes:
      1. Bacteriophage-Antibiotic Synergism to Control Planktonic and Biofilm Producing Clinical Isolates of Pseudomonas aeruginonsa. Faculty of Medicine, Alexandria University. On-going study.
      2. Effectiveness of Introducing Nucleic Acid Amplification Testing (NAT) for HCV Screening in Egyptian Blood Banks. Faculty of Medicine, Alexandria University. On-going study.
      3. Characterization of some Genotypic and Phenotypic Traits of Biofilm Producing Clinical Isolates of Methicillin Resistant Staphylococcus epidermidis (MRSE). Faculty of Medicine, Alexandria University. On-going study.
      4. Correlation between Bacterial Load of Helicobacter pylori Infection by Quantitative PCR and Histopathological Grade in Patients with Gastritis. Faculty of Medicine, Alexandria University. On-going study.
      5. Isolation of Aspergillus species from Respiratory Tract in Patients with Acute Exacerbation in Chronic Obstructive Pulmonary Diseases. Faculty of Medicine, Alexandria University. On-going study.
      6. Characterization of Acinetobacter strains isolated from patients in intensive care unit (ICU) in Alexandria main university and a study of their carbapenemases production. 2014
      7. The Relation of Helicobacter pylori to Obesity, Insulin Resistance and Thyroid Dysfunction. Faculty of Medicine, Alexandria University. On-going study.
      8. The Study of Platelets as a Potential Extrahepatic Reservoir for HCV. Faculty of Medicine, Alexandria University. June 2013.
      9. Detection of Helicobacter pylori in Children with Otitis Media with Effusion. Faculty of Medicine, Alexandria University. June 2013.
      10. Comparison between Antibacterial Coated and Non Coated Suture Materials after Surgical Removal of Impacted Mandibular Third Molar. Faculty of Dentistry, Alexandria University. February 2013.
      11. Molecular typing or methicillin resistant staphylococcus aureus clinical isolates on the basis of protein A and coagulase gene polymorphism. 2013
      12. Clinical relevance of serum antibodies positivity to six Helicobacter pylori antigens in patients with gastroduodenal diseases. 2013
      13. Prognostic role of Hepcidin level in chronic hepatitis C virus patients on Interferon therapy. 2013
      14. Shafiek HA, Abd-elwahab NH, Baddour MM, El-Hoffy MM, Degady AA, Khalil YM. Assessment of some Inflammatory Biomarkers as Predictors of Outcome of Non-Invasive Ventilation in Acute Respiratory Failure on Top of Chronic Obstructive Pulmonary Disease and Evaluation of the Role of Bacteria. International Scholarly Research Network; ISRN Microbiology, Volume 2012, Article ID 240841, doi:10.5402/2012/240841. http://www.isrn.com/journals/microbiology/2012/240841/cta/
      15. Molecular and serological techniques for diagnosis of culture negative infective endocarditis in Alexandria main university hospital. 2012
      16. Characterization of Burkholderia cepacia complex isolated from Alexandria main university hospital. 2012
      17. The use of in-vitro techniques to detect antimicrobial susceptibility patterns of isolates from cases of infective endocarditis. 2012
      18. Prevalence of Chlamydophila pneumoniae in patients with pneumonia in the main university hospital in Alexandria. 2012
      19. Penicillin bound nanoparticles in restoring the activity of beta- lactam antibiotics against methicillin resistant Staphylococcus aureus. 2012
      20. Polymerase chain reaction versus culture for detection of urogenital mycoplasmas in genital tract of infertile females. 2011
      21. Prevalence of Staphylococcus aureus and detection of superantigen genes among patients suffering from atopic dermatitis. 2009
  3. Training and education:
    • The lab provides training for master degree and doctorate degree candidates.
    • Training for medical technologists seeking a career in Diagnostic Microbiology.
    • In addition, the lab provides cyclic meetings for clinicians and nurses from different departments to highlight many issues such as proper sampling and antimicrobial resistance of different bacterial isolates.
    • Clinical Microbiology meetings are held regularly with staff members from the University hospital to tailor the best options for treating patients with infectious diseases.

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